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Strip harvesting is the most common technique for removing hair and follicles from a donor site. Doctor harvests a strip of skin from the posterior scalp, in an area of good hair growth. Scalpel is used to remove strip of hair-bearing tissue from the donor site. Incision is made in a way that intact hair follicles are removed. The excised strip is about 1.5 - 2 X 15–30 cm in size. From the strip excess fibrous and fatty tissue is carefully removed and dissected to individual follicular unit cells without damage to the follicular grafts, which are small, naturally formed groups of hair follicles, used for grafting.

Strip harvesting will leave a "pencil thin" linear scar in the donor area, which is typically covered by a patient's hair even at relatively short lengths. The recovery period is around 2 weeks and will require the stitches to be removed.